Pooja Makhija

5 Questions 9 Answers 0 Followers

Questions related from Pooja Makhija

Is there any standardized protocol for semi-dry transfer of 12% gel on pVDF membrane (biorad) using transblot system??

I see some of the ladder remaining on the gels at the low molecular weight side, so that raises my concerns.

06 June 2019 262 1 View

Can someone let me know if my cell cycle graphs are correct?

I am studying anticancer effect of plant extract on A549 cells using PI staining and BD LSR Fortessa flow cytometer. attached below is the image

04 April 2019 7,118 11 View

Why A549 cells show resistance on reaching p15 passages or more?

A549 cells around P5 to P10 passage were showing very good apoptotic trend in time and dose dependent manner with my plant extract in one set of data. When i tried to replicate data, in the...

04 April 2019 4,620 2 View

Does UV light of instrument affect UV absorbance?

My DPPH assay using plant extracts even ascorbic acid constantly reduces absorbance on keeping inside instrument, get stable value in 10-15 minutes?.

04 April 2018 10,045 5 View

How to determine solubility of a dried extract from plant material?

By extract here I mean hexane, dichloromethane, Tetrahydrofuran, methanol, water extracts of a known plant material

03 March 2018 9,205 1 View