Pooja Shree Mishra

5 Questions 20 Answers 0 Followers

Questions related from Pooja Shree Mishra

Why do I see a late amplification at ~37 cycles for the cytokine genes?

Hi! I am trying to standardise certain cytokines' m-RNA expression in glial cells, but the amplification happens at ~35-40 cycles. Considering that the cytokines are lowly expressed in...

08 October 2015 5,902 5 View

Can % viability be considered an accurate representation for proliferation measure by MTT assay?

My MTT assay shows proliferation in treated microglial cells as compared to noemal control. If one mesures using %viability, the values for the treated group come to 150% relative to the normal...

06 September 2015 6,210 12 View

Is 48 hrs time frame an optimal time point for studying Cytokine m-RNA and protein expression?

Hello! I am investigating the protein and m RNA expression of cytokines in microglia. Our protocol directs me to study both after 48 hrs of exposure, but i doubt if a time period that late could...

30 April 2015 8,009 11 View

Can anyone suggest why I do not see amplification in Real Time PCR?

I am trying to standardise my Tqman based qRT-PCR for TNF-alpha in Astrocyte cultures, but unable to do so. The starting primer and probe concentrations were 700 nM, tapered down to 300nM, to no...

15 January 2015 5,682 8 View

Cytokine assay - can anyone help?

Could someone suggest a highly sensitive, cost effective and efficient assay for estimating the secreted cytokines in body fluids or culture media. ELISA can be one alternative, but i have heard...

15 January 2014 2,383 7 View