How to prepare mice brain tissue homogenate for anti-oxidant (enzymatic and non-enzymatic) assays?

I work with mice and I prepared 10% (w/v) brain homogenates with 0.1M sodium phosphate buffer (pH 7.4). I centrifuge @ 10,000 rpm for 10 mins @ 4 degrees C. I use the supernatant for all the...

11 November 2013 8,254 4 View