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Questions related from Poly gen
Do anybody know how to get good mononuclear cell seperation from old blood (not much old, upto 2-3 days that take it for transport). Is there something to get rid off RBCs?
03 March 2011 7,749 9 View
I had a problem in western blot. Earlier i got gud band by the same procedure. But this time when i done with same procedure, and i got a gud signal on membrane after putting the...
01 January 2011 9,825 10 View
I am new to western blot and experienced that when i store my lysate at -20, and freeze thaw it , and run the gel, it works wel and gud band seen after developmentl, but after that if i repeat the...
01 January 2011 4,646 11 View
I want to lyse PBMCs. in that case do i need to put 100 microlitre for 1 ml? Is there is a variation of sigma cocktail according to cell type?
11 November 2010 4,009 6 View
