Polina Sutyrina

2 Questions 3 Answers 0 Followers

Questions related from Polina Sutyrina

How can I troubleshoot a failed plasmid sequencing reaction?

I've been doing some RF/Gibson cloning, and I am having issues sequencing my plasmids. I performed colony PCR to identify clones with the appropriate inserts. I then grew these up and plasmid...

22 October 2019 4,721 3 View

How can I troubleshoot a failed plasmid sequencing reaction?

I've been doing some RF/Gibson cloning, and I am having issues sequencing my plasmids. I performed colony PCR to identify clones with the appropriate inserts. I then grew these up and plasmid...

10 October 2019 4,809 4 View