Purification of recombinant protein cloned in pET28c using buffer 7.0 by Ni-NTA Agarose.

I have a cell-free extract in K phosphate buffer pH 7.0 and I want to run Ni-NTA column to purify my protein. The protocol says to use buffers of pH 8.0. Is it necessary? Can we try a different...

21 September 2012 3,096 20 View