Pijush Kanti Jhan

2 Questions 8 Answers 0 Followers

Questions related from Pijush Kanti Jhan

In western blot, how can I avoid stripping the nitrocellulose membrane?

I am trying to avoid stripping. For this reason I want to cut the membrane according to molecular weight and apply antibodies separately. Someone advised (picture attached) me to load the...

05 May 2018 2,380 6 View

How to extract protein from a tiny insect for SDS-PAGE?

Please help if you have any idea or experience. Normally I use 500 individuals of sweet-potato whitefly (Bemisia tabaci) for protein extraction in 1 ml RIPA buffer. I want to extract total...

03 March 2018 4,018 10 View