NEB HiFi Assembly started failing on all constructs, competent cells are fine. How do I solve this?

I am trying to assemble multiple constructs: - 2 fragments (1300bp and 2000bp) into a 3.3 kb vector - 1 fragment (2000bp) into 5 kb vector - another 2000bp fragment into the same vector - 1...

20 February 2019 1,925 11 View