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Hi I've been doing whole mount ISH on E9.5-10.5 mouse embryos (with DIG-labeled RNA probes). For a long time, I've been struggling with getting a decent signal before the background starts to...
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When reading the multitude of protocols for (W)ISH, most of these mention making PFA fresh from powder, but none that mention simply using commercial pfa solutions (e.g. 16% PFA from EMS, freshly...
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