Piero Lo Monaco

2 Questions 3 Answers 0 Followers

Questions related from Piero Lo Monaco

What luciferase protein concentration corresponds to a given luciferase activity?

Dual luciferase assay is an everyday well assessed technique in my lab. I use a bicistronic plasmid carrying two ORFs coding for Renilla luciferase and Firefly luciferase, respectively, and...

15 May 2017 8,226 9 View

How important is salt concentration in immunoprecipitation?

I'm working with a mRNA-ribosome-nascent polypeptide complex and I want to perform immunoprecipitation to purify the complex of interest (my nascent polypeptide bears an N-terminal tag). First, I...

31 January 2017 4,491 3 View