Pichia Pastoris

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Questions related from Pichia Pastoris

Why is there expression in shaker but not in fermenter?

I have a protein of interest that is expressing in Pichia. It is expressing well in the shaker flask, but when I apply the same expression methodology but now in a bioreactor I get minimal...

02 April 2018 6,209 7 View

Which insertion should I use in Pichia pastoris?

According to Invitrogen pichia pastoris cloning system using pPIC vectors there is an option to insert into AOX1 or HIS4 when using GS115 or KM71 strains. In both cases the SAME phenotype is...

25 October 2017 5,300 2 View

Why did my Pichia pastoris culture not grow?

I am using invitrogen manual for this with basal salt media and adding trace elements. Only thing I did not do is add biotin. Is that the problem or has anyone encountered other issues not...

27 September 2017 1,800 10 View