Phyo Wai Win

4 Questions 2 Answers 0 Followers

Questions related from Phyo Wai Win

Topo Cloning (pCR-XL-2-TOPO™ Vector)?

1:1 molar ratio of insert to vector is the best for performing Topo clone? Because I just put 1:1 molar ratio (insert to vector) and after 16 hr incubation with LB (kanamycin), I noted one colony...

12 March 2025 1,771 3 View

Why Avian influenza results are different by different target gene?

I am conducting Avian Influenza virus detection by real time rt PCR. I did 4 target AI genes and 4 primer probe sets for PCR. (H7 HA, H5 HA, NP and M target gene) I run real time 4 time...

05 April 2023 9,488 1 View

How do I calculate baser pair?

I have both forward, reverse and probe sequences and I want to calculate accurate base pair length to check band for PCR products. (Forward primer)nnnn(Reverseprimmer) is not work in blast. I...

10 March 2023 8,714 3 View

Why cross interaction come appear in multiplex real time PCR?

I am doing fourplex mqPCR panel for pathogens by Taqman probe . I did well choose for different dyes option (reporting dyes). real time PCR is five channel 7500 applied biosystem. For primary...

12 December 2022 872 4 View