Phuong Nghiem Thanh

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Questions related from Phuong Nghiem Thanh

Can I apply Michaelis menten kinetics to enzyme in fresh tissue homogenate ?

I'm measuring ADP ribose cyclase from fresh tissue homogenate using fluorescent detector ( microplane reader ). The graph shows the fluorescent of the product high at 0 mins, then decrease RFU,...

29 April 2021 1,463 3 View

Should I normalise data when measuring fluorescence intensity ?

I'm measuring fluorescence intensity of the product in an enzyme reaction in 37 celsius degree. I set a kinetic measurement in 1 hour. The fluorescence intensity of the BLANK ( contains only...

04 January 2021 5,745 5 View