Phillipe D. O'Brien

4 Questions 4 Answers 0 Followers

Questions related from Phillipe D. O'Brien

What is the optimal method of mouse euthanasia when investigating protein phosphorylation?

I'm interested in the insulin signaling pathway and want to identify markers of insulin resistance in DIO mice (e.g. pAKT/pIRS1/pJNK). Normally, we perform euthanasia by pentobarbital overdose...

13 September 2017 706 3 View

What is the ideal method for measuring mouse HbA1C?

Hello, I'm currently working in a lab that routinely assesses the metabolic profile of obese/diabetic-like mice. When measuring HbA1C in the past, we used a manual boronate affinity column...

19 April 2017 1,619 3 View

HFD-STZ NIDDM mouse model: Do insulin resistant HFD-fed C57BL/6J mice retain their insulin resistance post-STZ injection?

My question is related to the C57BL6 HFD-fed, streptozotocin-injected mouse model. Is there evidence indicating that these mice retain their insulin resistance post STZ-injection? In our model,...

16 September 2015 1,733 6 View

Seeking advice for preparation of lysates from mouse sciatic nerve & DRG for SDS-PAGE/WB.

I have recently started my first post-doc, switching from a pharmacology lab to a neurology lab, and am in the process of optimizing lysate generation from mouse nerve tissue as the current...

15 June 2012 7,643 3 View