Philippe Leone

3 Questions 3 Answers 0 Followers

Questions related from Philippe Leone

How can I avoid HEK cells from forming clumps?

I generated stable HEK lines, but some lines tend to clump (others not), and when I try to separate the cells by pipetting, lots of them dye. As I intend to run flow cytometry, I guess cell...

04 April 2013 6,273 4 View

Decrease antibiotic concentration after HEK293 stable selection?

I selected stable HEK293 clones with 0.4mg/mL Geneticin, now I am expanding them. Do I have to keep the same selective pressure or can I decrease the antibiotic concentration?

15 March 2013 2,908 4 View

HEK293 or 293T for stable line selections with Geneticin?

I want to select stable HEK with Geneticin in order to express a membrane receptor and run functional studies (overexpression is not important). I read that HEK293Ts are not the best cells for...

29 January 2013 8,257 11 View