Philip E Lapinski

3 Questions 6 Answers 0 Followers

Questions related from Philip E Lapinski

Best way to purify genomic DNA from human skin biopsy for long-range PCR?

I need to purify some genomic DNA from a human skin punch biopsy. In the past, I have done this with the Qiagen DNEasy Blood and Tissue kit. This yields good quality DNA for routine PCRs,...

24 July 2017 4,673 3 View

Is there an easy way to remove adipocytes from mouse tissue samples used for whole mount immunofluorescence?

We are trying to image mouse lymphatic vasculature by whole mount immunofluorescence, and these vessels are frequently associated with adipose tissue. The adipocytes obscure the view of the...

22 May 2015 6,438 3 View

Is it possible to effectively strip and reprobe tissues for whole mount immunofluorescence?

I routinely stain mouse diaphragms for immunofluorescence with antibodies against LYVE1 and CD31 to detect lymphatic and blood vasculature, respectively. These sections are mounted on slides with...

19 September 2013 4,904 8 View