Will the TrxA tag in the E. coli prokaryotic expression vector pET32a cause non-specific binding in WB or ELISA experiments?

Will the TrxA tag in the E. coli prokaryotic expression vector pET32a cause non-specific binding in WB or ELISA experiments? I used a fusion protein with TrxA to coat an elisa plate to test...

14 November 2023 8,565 1 View