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The JC10 dye measures mitochondrial membrane potential (MMP) which can be a measure of the viability of cells. However, I want to roughly assess OxPhos with JC10 (given that the MMP can be a...
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I knocked down a gene which has been shown to promote cell proliferation. qPCR results confirmed that the gene was indeed knocked down. There was increased expression of p53 as a result, which...
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My "no template" controls are showing products that are identical to their genes. I did bax and p53 qPCR and noticed that bax amplification was almost identical to bax no template control; p53,...
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I am using duplicate wells per sample in a 24 well plate. I had no problem when I extracted total RNA from a culture flask. I am using a Nucleospin extraction kit from Promega. I get low yields...
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Can I use RNAse-free water in the place of 'nuclease-free' water? Is RNase-free water always DNase-free OR is DNase -free water always RNase free?? The term nuclease-free water seems all...
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