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Questions related from Phil N Ubanako
I knocked down a gene which has been shown to promote cell proliferation. qPCR results confirmed that the gene was indeed knocked down. There was increased expression of p53 as a result, which...
25 September 2014 9,379 5 View
My "no template" controls are showing products that are identical to their genes. I did bax and p53 qPCR and noticed that bax amplification was almost identical to bax no template control; p53,...
27 May 2014 6,532 7 View
I am using duplicate wells per sample in a 24 well plate. I had no problem when I extracted total RNA from a culture flask. I am using a Nucleospin extraction kit from Promega. I get low yields...
04 September 2013 2,291 22 View
Can I use RNAse-free water in the place of 'nuclease-free' water? Is RNase-free water always DNase-free OR is DNase -free water always RNase free?? The term nuclease-free water seems all...
31 July 2013 893 4 View
