Phil Emnet

3 Questions 6 Answers 0 Followers

Questions related from Phil Emnet

HILIC mobile phase preparation?

I am new to HILIC method development, and have not been able to find a clear answer on how to prepare the necessary mobile phases. For example: Mobile phase: 10 mM ammonium formate in ACN/H2O...

30 January 2018 190 2 View

Can I derivatize C18 fatty acids in a polar solvent such as ethyl acetate?

I have aqueous samples containing (amongst other things) a mixture of C16-18 fatty acid methyl esters (FAMEs) which I extract using NaCl (7.5 g per 25 mL sample) and ethyl acetate. Other solvents...

03 January 2018 8,253 16 View

How to remove carry-over in LC-MS/MS gradient method when isocratic method shows no sign of carry-over

I have a new molecule for which I have initially developed an isocratic LC-MS/MS method (Synergi Fusion column, H2O/ACN 20/80 (+0.1% HCOOH)). Analysis of this method gives limited sensitivity...

26 June 2017 1,915 11 View