What's the procedure to get secreted hGM-CSF from recombinant S. cerevisiae BY4741?

I am trying to express hGM-CSF in S. cerevisiae. I use strain BY4741 and vector p427-TEF/MF-hGM-CSF. I've already gotten many recombinant clones (checked genotype) but I can't get secreted...

03 March 2014 3,882 17 View