Pham Ngoc Dung

5 Questions 18 Answers 0 Followers

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Can centrifuge in gradient density method separate the microplastics from flocs?

Hi everyone, I am working on how to separate microplastics (size = 100 um, density = 1.07 mg/ml) from flocs. I used sucrose 60% w/w (density= 1.28) as a medium in gradient density method to...

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How to detach biofilm from activated carbon?

Hi everyone, I intend to detach biofilm from activated carbon media by vortexing. I will then enrich bacteria. However, I am not sure how long and how strong that I should set up for vortexing...

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How to isolate species capable of degrading antibiotic (sulfamethoxazole) from activated carbon sample?

Hi every one, I have a problem in isolating bacteria capable of degrading sulfamethoxazole (SMX). My inoculum is granular activated carbon collected from lab biological activated carbon filter....

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How can I decide whether shannon index of two samples are significantly different or not?

Hi everyone, I have a small question. I have two samples data. Each sample contains around 55 cloned sequences. I use Mothur solfware to calculate Shannon index of 2 samples. Which hypothesis...

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How to calculate OTUs for the data of sanger sequences?

Hi every one, I run PCR, do DNA cloning, and then sequencing. I intend to use OTU to support information for taxonomic composition of the bacteria community. I see there are some way: - use the...

12 December 2014 6,915 21 View