Presence of homozygous indel changes in a cancer cell line after CRISPR Cas9 knock out?

Dear all, We have recently performed CRISPR Cas9 knocking out our gene of interest in the U87 glioma cell line. We used the pX459 plasmid from Addgene and tested various gRNAs (always only one...

10 October 2017 7,452 2 View