Peter Makram Ramzy

2 Questions 6 Answers 0 Followers

Questions related from Peter Makram Ramzy

What are the optimum centrifuge parameters for harvesting/cell clarification of yeast cultures [Hansenula Polymorpha]?

I would like to harvest Hansenula polymorpha [methylotropic yeast] culture using centrifugation. The optical density of the culture reaches 300 at 600nm [OD600 reaches 300 -350]. What are the...

08 August 2017 9,829 5 View

How can I inhibit or inactivate the proteases, which are secreted by Hansenula Polymorpha?

I am using the Hansenula Polymorpha for the production of rhPTH and I am suffering from the degradation of the PTH starting from 37 hour of fermentation duration and I am losing more than 50% of...

07 July 2016 7,806 6 View