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Questions related from Peter Wolfrum
Hello, i am a bit curious about my results. I transfected the human cell Line ARPE-19 with a Plasmid containing a GFP tagged p53 (p53-GFP, Plasmid #11770). After 24h i checked under the...
19 February 2021 4,007 7 View
Good day everybody, i am currently trying to transfect ARPE-19 cells with a GFP Plasmid (pEGFP-N1-FLAG; Plasmid #60360). I am using Viafect which works really well (transfection rate of over 95...
30 November 2020 3,070 6 View
Hello everybody, i hope somebody can help me with my cells. Since 2 weeks they started to change morphologically, as you can see in the two pictures with the smaller magnification (they started...
19 November 2020 4,864 5 View
19 November 2020 1,430 3 View
Goodday everybody, im currently trying to transfect primary porcine RPE Cells with a GFP Plasmid. So far i got ok-ish results (Maybe of around 30 % Transfection rate). How ever since the...
30 October 2020 9,304 3 View