Peter Wolfrum

5 Questions 7 Answers 0 Followers

Questions related from Peter Wolfrum

No mRNA increase in PCR after successful transfection in ARPE-19 cells ?

Hello, i am a bit curious about my results. I transfected the human cell Line ARPE-19 with a Plasmid containing a GFP tagged p53 (p53-GFP, Plasmid #11770). After 24h i checked under the...

19 February 2021 3,986 7 View

Why do my ARPE-19 Cells die after GFP-Plasmid (pEGFP-N1-FLAG) transfection?

Good day everybody, i am currently trying to transfect ARPE-19 cells with a GFP Plasmid (pEGFP-N1-FLAG; Plasmid #60360). I am using Viafect which works really well (transfection rate of over 95...

30 November 2020 3,055 6 View

Primary porcine RPE cells look abnormal ?

Hello everybody, i hope somebody can help me with my cells. Since 2 weeks they started to change morphologically, as you can see in the two pictures with the smaller magnification (they started...

19 November 2020 4,845 5 View

Whats wrong with my cells ? contamination ?

Hello everybody, i hope somebody can help me with my cells. Since 2 weeks they started to change morphologically, as you can see in the two pictures with the smaller magnification (they started...

19 November 2020 1,411 3 View

Transfection does not work anymore (Has worked before) ?

Goodday everybody, im currently trying to transfect primary porcine RPE Cells with a GFP Plasmid. So far i got ok-ish results (Maybe of around 30 % Transfection rate). How ever since the...

30 October 2020 9,297 3 View