Peter Kan

7 Questions 3 Answers 0 Followers

Questions related from Peter Kan

Can RNase or DNase survive in pure chloroform >99%? or they will be denatured?

I recently found an old bottle of opened chloroform (2.5L) in our lab, we would like to use it for RNA extraction, but not sure if it has been used for other experiments and would be exposed to...

27 September 2024 8,138 3 View

Can DEPC-treated water be used as a replacement for OCT when embedding frozen tissue on cryostat?

Hi everyone, I am currently working on RNA extraction on tissue, but unfortunately, I only have large frozen clinical tissue on hand. In order to maximize the RNA integrity, I plan to use...

26 June 2024 7,608 1 View

Does Trizol LS yield more and better RNA than normal Trizol?

Hi, I am currently working on RNA extraction on blood serum samples using Trizol. However, the RNA yield is quite bad, on average, only yielded 200ng per 200ul of sample input, and the A260/280...

19 June 2024 7,239 3 View

Which miRNA extraction is better? TRIzol or Qiagen miRNeasy kit?

Hi, I am currently looking for a reliable method to extract miRNA from cell and serum samples. I have read some papers stating TRIzol is suitable for both samples, but the result highly depends...

25 April 2024 4,059 3 View

External or internal threaded cryovial, which one is better for cell cryopreservation?

Hi, I am currently looking for suggestions on choosing which cryovial to use for cell cryopreservation, internal or external threaded, do you guys have a preference? or is there any reason for...

12 March 2024 1,634 1 View

Can I use the product cDNA from Qiagen miRNA LAN RT kit with other brand SYBR green master mix?

I am currently doing miRNA research and got the miRCURY LNA RT kit from Qiagen for RT reaction to turn my RNA into cDNA. A few papers have used SYBR green kit from another brand and not the...

11 March 2024 398 1 View

Can I amplify cDNA with PCR for qPCR, and still get a reliable result?

Hi guys, I am currently working on qRT-PCR. However, the amount cDNA I got from RT reaction is too little to assess all the targets. I saw some papers and websites mentioning amplifying cDNA with...

01 March 2024 174 3 View