Peter Victorovich Dubovskii

4 Questions 11 Answers 0 Followers

Questions related from Peter Victorovich Dubovskii

How to optimize conditions for labeling of a hydrophobic peptide with Cy3(5)-maleimide dye?

Hydrophobic peptide (mass=1 mg), 30 a.a.-long contains a single Cys-residue, can be dissolved in trifluoroethanol. I need to attach Cy3 (or Cy5) maleimide to the thiol group of the peptide. What...

03 July 2013 9,759 3 View

Cell-free or bacterial expression? Which of the methods is advantageous in obtaining a recombinant disulphide-rich protein?

The protein is 60-residue long and possesses 4 disulphide bonds.

01 April 2013 4,361 5 View

How to determine the volume of a molecule? Is there a convenient program for that?

Not only proteins or peptides but also low-molecular weight compounds, like calcein.

23 January 2013 6,425 9 View

How precise is it to estimate helicity in a peptide from ellipticity at 220 nm (CD-spectroscopy)?

Peptide, 26 aa (GLFGKLIKKFGRKAISYAVKKARGKH), water/TFE mixture.

14 January 2013 3,756 3 View