Peter Victorovich Dubovskii

7 Questions 15 Answers 0 Followers

Questions related from Peter Victorovich Dubovskii

How to optimize conditions for labeling of a hydrophobic peptide with Cy3(5)-maleimide dye?

Hydrophobic peptide (mass=1 mg), 30 a.a.-long contains a single Cys-residue, can be dissolved in trifluoroethanol. I need to attach Cy3 (or Cy5) maleimide to the thiol group of the peptide. What...

03 July 2013 9,802 3 View

Cell-free or bacterial expression? Which of the methods is advantageous in obtaining a recombinant disulphide-rich protein?

The protein is 60-residue long and possesses 4 disulphide bonds.

01 April 2013 4,406 5 View

What is the highest content of phosphatidylserine in the outer envelope of the plasmatic membrane of cancer cells?

Are these cells HL60?

01 February 2013 463 0 View

Normal Mode Analysis (NMA) of protein mobility: how useful is it and are there means to do it quickly?

Time-scale of NMA?

31 January 2013 3,149 8 View

How to determine the volume of a molecule? Is there a convenient program for that?

Not only proteins or peptides but also low-molecular weight compounds, like calcein.

23 January 2013 6,467 9 View

Is there a good program to calculate (predict) 13C-chemical shifts of low molecular weight compounds (e.g. calcein)?

Calcein is a fluorescent dye (http://en.wikipedia.org/wiki/Calcein).

18 January 2013 7,975 8 View

How precise is it to estimate helicity in a peptide from ellipticity at 220 nm (CD-spectroscopy)?

Peptide, 26 aa (GLFGKLIKKFGRKAISYAVKKARGKH), water/TFE mixture.

14 January 2013 3,798 3 View