Peshala Eranga Gunasekara

6 Questions 13 Answers 0 Followers

Questions related from Peshala Eranga Gunasekara

What would be the best vector graphic software for a scientific drawing?

I am searching for an open source software which can be used for scientific drawings.

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Does KU 812 cell line show a self differentiation to mature basophils?

I want to differentiate KU 812 premature basophils to mature basophils. What would be the best way to differentiate this cell line?

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Why protein bands are not detect in Western blots?

I have recently performed a Western blot with the aim of detecting some allergens. Clear bands were resulted in SDS-PAGE and complete transfer of the bands to the membrane (NCP) was observed. I...

08 August 2017 2,736 8 View

How could I evade the protein aggregation in SDS-PAGE gel electrophoresis?

I have performed a SDS-PAGE using wasp venom and most of the proteins remained on the top of the well without being gone through the gel. Is there any specific reason for this aggregation? and how...

08 August 2017 4,455 16 View

What would be the alternative alkylating agents other than iodoacetamide which I can use in amino acid sequencing?

Iodoacetamide, is an alkylating agent. It reacts with cysteine residues in proteins. It is often used to modify SH-groups to prevent the re-formation of disulfide bonds after the reduction...

07 July 2017 4,882 5 View

Can perform the basophil activation test using healthy individuals' basophils?

I am looking for an alternative way of doing basophil activation test(BAT) using passive sensitization. Technically, sensitizing the besophils from the healthy individuals' by patients sera and...

01 January 1970 8,760 4 View