Penelope Kastana

4 Questions 4 Answers 0 Followers

Questions related from Penelope Kastana

Why OD260 or OD280 is so high in nanodrop when I extract RNA from eucaryotic cells?

Hi everyone! I would like some help about purified RNAextraction using the PureLink RNA MiniKit. I followed the protocol that the company suggests for 800000 cells. I had 399ng/μl concentration...

24 July 2018 866 4 View

RNA isolation from mice tails?

Hi!!!I'm currently trying to isolate RNA from mice tails (with the NucleoSpin kit from Macherey-Nagel) in order to genotype them. But the concentration I measure in nanodrop is 5-9 ng/ml and even...

27 July 2016 8,050 4 View

Why does the shape of HUVEC change in P1?

I have isolated HUVEC and in P0 they were just like they should be(like cobblestones).In P1 they became elongated and they don't look like HUVEC anymore. I had thought that this was because of the...

14 June 2016 2,332 3 View

High background and white spots in my blots?

Hi!I have recently encountered problems with the Western blot analysis. I get blots with high background(all black!) and some white spots. After stripping and using the same antibody, I had also...

01 June 2016 7,950 9 View