Penchit Chitnumsub

5 Questions 4 Answers 0 Followers

Questions related from Penchit Chitnumsub

What causes protein skin or thin film and How to prevent the skin in the crystallization drop?

We observed thin film covering the protein drop within 6 days at 22 C incubator in the microbatch plate, using 1 microliter of protein and 1 uL of crystallizing agent per drop, covering with oil....

19 July 2025 5,872 0 View

How do you explain the differences in protein molecular mass among techniques?

My protein showed heptamer in SEC but pentamer in SEC MALS, and dimer in SV-AUC. How do you explain the difference in these molecular mass among these techniques?

27 May 2025 6,250 4 View

What makes the protein not bind both anion exchange at Tris buffer pH 8 + 10% glycerol and cation exchange at Mes pH 6 + 10% glycerol?

Why does the protein not bind both anion exchange at Tris buffer pH 8 + 10% glycerol and cation exchange at Mes pH 6 + 10% glycerol? Any suggestion for purification of this tag-free protein. The...

01 June 2020 9,416 0 View

Why did my crystal stop crystal growing after a few days?

The crystallization was set up at 22 degree C using microbatch method with a ratio of protein/buffer=1. A protein crystal appeared in three days without further growth in a couple days even though...

08 May 2015 4,407 4 View

Problem with the acidic pH of PEG solution: how can we make high pH PEG1500 stock solution (pH 9) without damage?

How can we make a crystallizing buffer at basic pH while the acidic PEG solution decreased the final pH of the solution? We optimized a crystallizing solution from 0.1M SPG buffer+25%PEG1500 in...

09 January 2015 6,306 8 View