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I use hygromycin gene primers to do PCR for ICMB 01222 pearl millet wild type and get a strong band. After sequencing, the sequence is hygromycin gene. I feel very confused. I also use water as a...
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Pour an agar plate without PEG first and then put a PEG-containing overlay solution on top of the solidified agar. I see this method on verslues et al., 2006. But this method is more than 10 years...
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