Pedro de los Reyes Rodríguez

6 Questions 8 Answers 0 Followers

Questions related from Pedro de los Reyes Rodríguez

Several questions about my ChIP experiment using IgG beads. Can anybody help me?

Hello! I am trying to do a ChIP experiment using the IgG sepharose beads from GE Healthcare. I work with a recombinant protein in Arabidopsis fused to TapTag (Calmodulin binding peptide,...

01 January 2018 4,132 2 View

How to improve the RNA extraction in Ostreococcus tauri?

Hi to all. I have some problems in extracting RNA from Ostreococcus tauri through the Trisure protocol. In this protocol, I collect the pellet of 25 ml from Ostreococcus culture by centrifuging...

02 February 2017 1,383 2 View

Which is the best way for visualizing a big network in Cytoscape?

I'm using Cytoscape for visualizing my transcriptional network, but it is huge. Specifically, it has 15842 nodes and 49812 edges. I have tested the prefuse force directed layout, is there a...

03 March 2015 3,174 20 View

How can I convert RSEM gene IDs to gene names?

I have developed a RSEM analysis in order to get a differential expression matrix. I have obtained a matrix of a list of genes with this kind of ID: c48895_g2. I can't realised a enrichment of GO...

02 February 2015 2,759 7 View

How can I analyze a set of DEGs (differentially expressed genes) to obtain information from them?

I have obtained a set of DEGs after making de-novo RNA-seq data analysis with Trinity. I've already made the annotation of the DEGs using KAAS. What other tests can I do? Thank you

02 February 2015 5,334 10 View

What is your criteria for choosing the target genes in ChIP-seq?

What do you do when a peak overlaps a gene? Do you choose these gen as a target?

12 December 2014 4,230 3 View