Pedro P Cunha

6 Questions 11 Answers 0 Followers

Questions related from Pedro P Cunha

Can I compare 2 groups from independent experiments with different baseline readings by normalizing to the average of the controls of each experiment?

Apologies if this has been answered, I was not able to find anything similar to my question. In 3 separate days, I have analysed cells from 10 WT and 10 KO animals by flow cytometry. Below I show...

14 September 2021 3,593 4 View

Should I coat the wells and starve the cells for a scratch assay with Glioblastoma cell lines?

Helo dear colleagues After transfection and a 48h drug treament we want to assess the motility of our Glioblastoma cell lines. Is it important to use a ECM coating to perform this assay? (during...

13 June 2015 786 3 View

What is the most correct formula to assess cell viability with Alamar Blue assay?

We add resazurine to the following conditions: Just media (neg Control) Non-transfected cells (our positive control) Non-transfected cells + drug Transfected cells Transfected cells + drug To...

21 February 2015 2,267 6 View

Is there any reliable program that designs primers for long non-protein coding RNAs?

What we pretend are primers for qRT-PCR. The secondary structure of ncRNAs makes it difficult to design primers with regular programs such as Beacon ins't it?

13 February 2015 443 4 View

What is the current gold standard technique for the in vitro pro-angiogenic features of cancer cells?

In my lab we are interested in fighting glioblastoma pretty hard. Now we are using two pan-tyrosine kinase inhibitors, namely Sunitinib and Axitinib. Because one of the major in vivo tumor...

30 January 2015 7,046 9 View

Is there any technique to evaluate stem cells self-renewal/assymetric division besides the microscopial ones?

I'm currently working with isolated Glioblastoma stem-like cells and in order to make sure they are in fact what I think they are, I would like to see if they indeed divide assymetrically. I now...

30 January 2015 7,285 5 View