Payal Barua

2 Questions 6 Answers 0 Followers

Questions related from Payal Barua

Which percentage of SDC would be helpful for eluting the biotinylated protein from the neutravidin beads?

I use Sulfo-NHS-S-S biotin as a labeling reagent and pierce neutravidin beads for capturing. I have used 50mM DTT at RT before. But its not working. Now I want to try SDC and TEAB with DTT and...

13 June 2017 9,600 3 View

Have anyone used 'Dounce' homogeniser for cell lysis? Can't reproduce the protein amount got by it. How to resolve this?

Used Hepes buffer with 100 strokes of Dounce, still protein recovery is low.

30 March 2017 8,352 4 View