Pawan Singh

2 Questions 31 Answers 0 Followers

Questions related from Pawan Singh

Which lysis buffer should be used for HeLa cells to detect an actin bound protein by western blot?

I am trying to detect a 3XFLAG-tagged bacterial protein by western blot which has been transfected in HeLa cells. But when I lyse the cells by RIPA buffer I am not able to detect the protein by...

01 January 2015 654 12 View

Can anybody explain why my protein is not entering SDS gel in non-denaturing conditions?

I am doing SDS page for a purified bacterial protein, His-tagged at N-terminal in non-denaturing conditions (buffer final concentrations: 40 mM Tris/HCl pH 6.8, 4% glycerol, 0.64% SDS, 0.01%...

11 November 2013 1,612 31 View