Pawan Basniwal

5 Questions 3 Answers 0 Followers

Questions related from Pawan Basniwal

Formic acid improve the quality of bands in HPTLC. How it works? What is chemistry?

Generally, formic acid improve the quality of the bands of components in HPTLC. How it works? What is the chemistry behind it?

05 December 2019 9,585 3 View

How can we separate E and Z isomer on C18 column (non-stereospecific)?

How can we separate E and Z isomer on C18 column (non-stereospecific) with using specific mobile phase.

09 October 2014 4,843 1 View

Why does sodium adduct abundance appear in mass spectrum?

When fragmentation of the molecule is analysed, then sodium adduct is normally observed with parent ion. Why is it so?

20 June 2014 431 7 View

Can methanol (as a mobile phase component) react with the free phosphate group of an analyte, where 0.1% formic acid is aqueous part of mobile phase in HPLC?

The drug has a free phosphate group; mobile phase 0.1% formic acid and methanol in HPLC. Is it possible that methanol can form an ester with the free phosphate group of the drug (analyte)?

05 February 2013 3,218 1 View

If triethylamine is used with a C18 column for a prolonged period, what will happen to the C18 column?

Triethylamine interacts with silanols, but when it is used regularly with a C18 column, I think there may be an effect on the life of the column.

31 January 2013 9,352 4 View