Pavithra Anantharaman Sudhakari

4 Questions 13 Answers 0 Followers

Questions related from Pavithra Anantharaman Sudhakari

Why am I getting this, '[E::sam_parse1] no SQ lines present in the header' error?

I need to take multiple FASTA files as reference genome and for each reference genome, I need to do reference-based assembly. Here is my code, import os import glob from...

30 September 2021 3,350 1 View

Why am I not getting any plaques while doing top agar method?

When I spot the phage lysate on MG1655, I see the clearance. But, to calculate the phage titer I mix the lysate (100ul) with MG1655 as background strain (100ul) in 3ml 0.5% agar. In this case, I...

17 August 2021 7,197 21 View

What is the growth rate of a bacterial cell carrying a toxin-antitoxin system containing plasmid?

How does this growth rate different from that of wild-type containing no plasmid?

12 August 2021 1,255 5 View

What does "1-log loss of viability" mean in bacterial growth experiments? How can it be interpreted in terms of death probability or death rate/hr?

I am looking for the death rate of a bacterial cell due to plasmid loss in case of post-segregational killing (toxin-antitoxin system containing plasmids).

12 August 2021 4,283 1 View