Paulo Caldas

2 Questions 4 Answers 0 Followers

Questions related from Paulo Caldas

How can the addition of ATP and MgCl2 improve a protein purification procedure? Is it useful to decrease salt concentration in some cases?

This is a very general question, I know, but I've been purifying proteins and exploring expression/purification conditions in E.coli during the last couple of months and I'm having trouble to find...

10 October 2016 6,019 5 View

How does glycerol increase protein stability and solubility?

Using imidazole and glycerol in the protein purification process can increase protein stability and solubility. What is the chemical mechanism for that?

07 July 2015 6,502 5 View