Do 40bp homology primers (H1 and H2) work for Datsenko (2000) methodology for deletion genes in E. coli?

Datsenko (2000) demonstrated one-step inactivation of chromosomal genes using primers with 36- to 50-nt extensions that are homologous to regions adjacent to the gene to be inactivated (H1 and H2)...

18 March 2014 2,804 2 View