5 Questions 8 Answers 0 Followers
Questions related from Paula Maguire
Hi all, I am trying to detect the levels of RORC in my cells. I am using a probe from Merck and my housekeeping gene is GAPDH. I am getting GAPDH when I perform the qPCR, but no RORC. This...
04 April 2019 4,389 2 View
I've been trying to detect IL-12p70 from supernatants from PBMCs, however I keep getting negative absorbance values. I've been using the DuoSet from R&D. But, I was wondering if anyone else...
07 July 2016 8,687 14 View
I'm planning on making my own MACS buffer, but I was wondering how long it can be stored for and under what conditions? I am waiting on some of my supplies to be delivered so I don't want to waste...
10 October 2015 232 0 View
I'm looking to investigate if my protein (IRF3) is in monomeric or dimeric form. In order to effect some of its functions, I know that IRF3 must be phosphorylated and form a homodimer. So, I want...
01 January 1970 8,056 8 View
I recently received new stocks of influenza virus - the concentrations are in HA titres. I was just wondering if there was some calculation that I could do in order to convert the HA units into...
01 January 1970 4,665 12 View
