Paula Vila Gomez

2 Questions 4 Answers 0 Followers

Questions related from Paula Vila Gomez

How can I improve subcellular fractionation?

I need to obtain nuclear and cytoplasmic fractions from my cultured cells (HEK293AAV) but I cannot seem to get pure fractions. I am using the nuclei EZ kit. I am following the manufacturer's...

23 March 2021 3,184 0 View

Questions about frozen tissue sectioning and fixing for H&E?

Hi all, I am planning some in vivo work in which I will do immunofluorescence, immunohistochemistry, and H&E staining on OCT-embedded unfixed frozen mouse tissue. I have read many different...

11 August 2020 1,460 7 View