Paula Maguire

2 Questions 6 Answers 0 Followers

Questions related from Paula Maguire

Is it necessary to perform a DNase treatment when using the RNeasy Mini Kit during RNA isolation?

I have been isolating RNA from CD14 monocytes purified from PBMCs but the yield has been low. I've read that DNase treatment can result in lower RNA yields, but I want to ensure that these results...

06 April 2017 4,066 4 View

What is the best ELISA kit for detecting Human IL-12p70?

I've been trying to detect IL-12p70 from supernatants from PBMCs, however I keep getting negative absorbance values. I've been using the DuoSet from R&D. But, I was wondering if anyone else...

28 July 2016 9,124 13 View