Paula Garcia-Fraile

2 Questions 7 Answers 0 Followers

Questions related from Paula Garcia-Fraile

How might one amplify bacterial 16S from a DNA mixture with a minority of bacterial DNA?

I am trying to amplify 16S from DNA obtained from wood samples. My aim is to study microbial endophytes. It is supposed that the majority of the DNA corresponds to the tree DNA. I can amplify with...

07 November 2014 6,876 6 View

Any tips for resuspending clumps of DNA?

I am working with some DNA samples obtained with a phenol/chloroform extraction and I have many clumps that I can't resuspend in the buffer (TE). I tried heating for 10 min at 42C and with the...

09 May 2013 5,900 10 View