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We are studying the kinetics of a microbial enzyme, and have run across an interesting phenomenon I have not observed before. In attempting to establish a Vmax and Km for the enzyme by varying...
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We already have in place HPLC/UPLC based methods, but these are ' stopped time' based, so tedious for kinetic studies. Looking for a colorimetric assay (eg. enzyme coupled?) that would target...
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