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I'm currently trying to use the method outlined in this paper to add a micrometer scale pattern over a nanopillar array, the final application being tissue engineering. My substrates are...
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Can anyone with experience using a microtome suggest whether it's possible to use one to cut 100-220 micron slices from a plastic (polycarbonate) piece? I'd like to cut the surface from a plastic...
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I've been having some difficulty immunolabelling talin to look at focal adhesions - wide field fluorescence at 100x being the imaging method. My standard fixation process is 10 mins at 37C in 4%...
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