Can a wrong input of RNA and standard curve in a qPCR, resulting in lower RNA concentrations in the mix, be corrected afterwards?

I performed a qPCR in which I had an input of 5uL RNA as well as the standard curve instead of the 10uL that was supposed to be the input. The mix (15uL) that was made was based on a 10uL input....

04 December 2020 8,807 1 View