Paul Naphtali

4 Questions 2 Answers 0 Followers

Questions related from Paul Naphtali

Can I use a hydrogel-encased sample as flow cytometry input?

I'm currently thinking about using flow cytometry to characterize and isolate individual cells from my sample that's encased in a hydrogel. This way, I can examine microbial growth rates among...

06 June 2017 5,702 1 View

Growth Curve Aseptic Technique

Hi all, I'm going to make a growth curve by transferring 1-mL volumes from 25 mL of flask liquid culture into a cuvette to measure OD600. I don't want to keep throwing away the contents inside the...

06 June 2017 4,290 1 View

Why do I not see any amplification after PCR?

Hi all, I am currently troubled with my PCR assays because what used to be a successful amplification and visualization of my amplicons has suddenly stopped working. Here is the following protocol...

01 January 2015 4,931 10 View

Why are there uneven temperature intervals after setting the temperature range on the T100 thermal cycler?

I am currently trying to set up a gradient PCR to determine the optimal annealing temperature for my 16S primer pair. Our lab runs all our PCRs with the BIO-RAD T100 thermal cycler. This machine...

11 November 2014 6,343 2 View