Paul Maciocia

2 Questions 3 Answers 0 Followers

Questions related from Paul Maciocia

BrdU assay - how stable are cell lysates frozen prior to analysis?

I have run a mixed lymphocyte reaction assay, using PBMCS and added BrdU after 5 days prior to reading out the assay by a BrdU ELISA today (12-24 hrs after adding BrdU). Unfortunately the ELISA...

01 January 2018 9,756 0 View

How do I compare the relative expression of 2 genes by qPCR, across different samples?

My experiment is this: there are 2 very similar genes which are expressed in a mutually exclusive fashion but at essentially identical levels. I want to look at normal donor and primary samples...

01 January 2016 3,369 2 View