Paul Naphtali

3 Questions 4 Answers 0 Followers

Questions related from Paul Naphtali

Growth Curve Aseptic Technique

Hi all, I'm going to make a growth curve by transferring 1-mL volumes from 25 mL of flask liquid culture into a cuvette to measure OD600. I don't want to keep throwing away the contents inside the...

22 June 2017 7,204 1 View

Why are there uneven temperature intervals after setting the temperature range on the T100 thermal cycler?

I am currently trying to set up a gradient PCR to determine the optimal annealing temperature for my 16S primer pair. Our lab runs all our PCRs with the BIO-RAD T100 thermal cycler. This machine...

28 November 2014 3,103 2 View

How can I optimize the flow rate of my water sample and standard nitrate solutions through the cadmium reduction column?

Hi all, I am currently attempting to formulate a publication-quality standard curve for the quantification of nitrates in a water sample with the cadmium reduction method. I have purchased a...

25 November 2014 6,288 5 View