Paul G Dennis

3 Questions 7 Answers 0 Followers

Questions related from Paul G Dennis

Can I use 18S sequences corresponding to top blast hits for fungal ITS2 seqs to build a phylogeny

18S rRNA genes in fungi can be used to discriminate between different classes or orders, but not to family-species level, which is why we use ITS. The problem though with ITS is that we cannot...

25 September 2014 6,940 3 View

What is the coarsest 'taxonomic' level that fungal ITS2 phylogenies can be built at?

I would like to generate phylogenetic distance metrics for groups of ITS2 sequences from different geographical locations. Obviously it is not possible to make an 'all fungi' phylogeny with ITS2...

25 September 2014 1,484 4 View

Does anyone have a good idea for a soil microbiology lab practicals aimed at 2-3rd year honors students?

I need something cheap (so PCR is out of the question for now). I was thinking about something on biogeochemical cycles. Winogradsky columns or perhaps trying to form pyrite then reoxidising the...

11 May 2013 3,550 3 View