Patryk Firmanty

2 Questions 1 Answers 0 Followers

Questions related from Patryk Firmanty

How concentrated are the lactate stocks you prepare?

Hi, I am using 20 mM lactic acid to induce macrophages polarisation. I have L-(+)-lactic acid from Sigma and in their protocol the solubility of the powder is 100 mg/ml H2O. I am using PBS instead...

24 January 2025 5,393 0 View

How could I improve monocyte derived macrophages detachment using accutase?

Hi! I am doing flow cytomety experiments on monocyte derived macrophages. I tried to use accutase which should not affect macrophages surface markers. However, I can detach no more than 50% of...

15 December 2024 6,852 2 View